Izindlela zokutholwa kwe-disodium-5-ribonucleotide (IMP + GMP)

Kunezindlela ezimbili eziyinhloko zokutholwa kwe -disodium-5-ribonucleotide (IMP + GMP) : QB / T 2845-2007 ukwengeza ukudla —Disodium-5-ribonucleotide (IMP + GMP) kanye ne-SB / T 10371-2003 ingqikithi yenkukhu.

Izindlela ezimbili zokulinganisa ezijwayelekile zisebenzisa i-spectrophotometry, kodwa izindlela ezithile zokulinganisa zihluke kakhulu

I-QB / T 2845 isithako sokudla - i-disodium-5-ribonucleotide (IMP + GMP), yamukelwe

Indlela ye-wavelength ekabili [1]: kala u-0.4000 (m) g wesampula, chitha futhi ulungise ivolumu ngamanzi

Ku-250 ml, ncela u-5.0 ml, bese ulungisa ivolumu nge-0.01 mol / L hydrochloric acid ukuze

250 ml, ne-0.ol mol / L njengento engenalutho, ama-absorpnce A no-B ku-250 nm no-280 nm alinganiswa nge-cuvette engu-10 mm. I-Disodium-5-ribonucleotide (IMP + GMP) iqukethe

Inani libalwa ngokwefomula (1).

ω Ingabe iphesenti lomswakama lokuqukethwe kwe-nucleotide disodium yokunambitha.

I-Sb / T 10371 isebenzisa indlela eyodwa yobude beza.]: Isampula yesisindo esingu-2 ~ 4G

(m) yahlakazwa ngenani elincane le-0.01 mol / L hydrochloric acid futhi yalungiswa ku-100 ml,

Hlunga, ncela u-5.00 ML kuya ku-100 ml weflask yevolumu futhi usebenzise

0.01 mol / L i-hydrochloric acid kuya kuvolumu. Sebenzisa 10 mm cuvette, 0.01 mol / L

I-Hydrochloric acid yayisetshenziswa njengento engenalutho ukukala ukumunca kwayo.

Lezi zindlela ezimbili zisetshenziswa njengamanje, futhi awukho umngcele oqinile endaweni yesicelo

Umkhawulo. I-Sb / T 10371 iyindlela yokuthola ingqikithi yenkukhu, ikakhulukazi ehloswe ngayo

Ukutholwa kokuqukethwe kwe-I + G: okuqukethwe cishe ku-1 ～ 39,6, kanye ne-QB / T 2845

Indlela yokuthola izithasiselo ezihlanzekile ze-I + G, kodwa kwezinye eziqukethe i-I + G

Ibuye isetshenziswe emikhiqizweni enenani eliphansi, njenge-GB / T 8967

Okuqukethwe kwe-I + G emafutheni acwengisisiwe kukalwa ngokucaphuna okuqukethwe kwe-I + G ku-QB / T 2845

Indlela yokuthola. Ngenxa yalesi simo, umbhali uthathe ingqikithi yenkukhu njengesampula waqhathanisa kokubili

Ucwaningo lokuqhathanisa lwalezi zindlela ezimbili lwenziwa ukuze kuphenywe ukufana nokuhlukana kwazo, ukuze kwenziwe olunye ucwaningo mayelana nenqubo yocwaningo.

Ihlinzeka ngereferensi yokwakhiwa kwamazinga okunqunywa kwe-I + G.