Nzira dzekuona dze disodium-5-ribonucleotide (IMP + GMP)

Pane nzira mbiri huru dzekuona disodium-5-ribonucleotide (IMP + GMP) : QB / T 2845-2007 kuwedzera kwechikafu -Disodium-5-ribonucleotide (IMP + GMP) uye SB / T 10371-2003 essence yehuku.

Iwo maviri akajairwa nzira dzekuyera anotora spectrophotometry, asi chaiyo yekuyera nzira dzakasiyana zvakanyanya

QB / T 2845 chikafu chekuwedzera - disodium-5-ribonucleotide (IMP + GMP), yakagamuchirwa

Dual wavelength nzira [1]: kuyera 0.4000 (m) g yemuenzaniso, nyungudutsa uye gadzirisa vhoriyamu nemvura.

Kusvika 250 ml, yamwa 5.0 ml, uye gadzirisa vhoriyamu ne 0.01 mol / L hydrochloric acid kusvika

250 ml, ine 0.ol mol / L isina chinhu, iyo inotorwa A uye B pa250 nm uye 280 nm yakayerwa ne10 mm cuvette. Disodium-5-ribonucleotide (IMP + GMP) ine

Huwandu hunoverengerwa maererano neformula (1).

ω Ndiyo chikamu chemwando chemukati chekuravira nucleotide disodium.

Sb / T 10371 inotora nzira imwe chete yewavelength.]: Kuyera 2 ~ 4G sampuli

(m) yakanyungudutswa nehuwandu hushoma hwe 0.01 mol / L hydrochloric acid uye yakagadziriswa ku100 ml,

Sefa, yamwa 5.00 ML kusvika 100 ml volumetric flask uye shandisa

0.01 mol / L hydrochloric acid kusvika kuhuwandu. Shandisa 10 mm cuvette, 0.01 mol / L

Hydrochloric acid yakashandiswa sechinhu chisina chinhu kuyera kunyura kwayo.

Idzi nzira mbiri dziri kushandiswa pari zvino, uye hapana muganhu wakasimba mundima yekushandisa

Limit. Sb / T 10371 ndiyo nzira yekuona yehuku essence, kunyanya kunanga

I + G yekuona zvemukati: zvirimo zvinenge 1 ～ 39,6, uye iyo QB / T 2845 iri.

Iyo nzira yekuona yakachena I + G yekuwedzera, asi mune imwe I + G ine

Inoshandiswawo mune zvigadzirwa zvine yakaderera huwandu, senge GB / T 8967

Zviri mukati meI + G mumafuta akacheneswa zvinoyerwa nekutaura zvirimo muI + G muQB / T 2845.

Nzira yekuona. Nekuda kwemamiriro ezvinhu aya, munyori akatora hupfu hwehuku semuenzaniso ndokuenzanisa zviviri izvi

Chidzidzo chekuenzanisa chenzira mbiri idzi chakaitwa kuongorora kufanana kwavo nekusiyana kwavo, kuitira kuti paitwe imwe chidzidzo pa

Inopa chirevo chekuumbwa kwemazinga ekutemerwa kweI + G.