Iindlela zokujonga i-disodium-5-ribonucleotide (IMP + GMP)

Kukho iindlela ezimbini eziphambili zokufumanisa i- disodium-5-ribonucleotide (IMP + GMP) : QB / T 2845-2007 ukongezwa kokutya-Disodium-5-ribonucleotide (IMP + GMP) kunye ne-SB / T 10371-2003 inkukhu essence.

Iindlela ezimbini zokulinganisa umgangatho zisebenzisa i-spectrophotometry, kodwa iindlela ezithile zokulinganisa zahluke kakhulu

I-QB / T 2845 isongezo sokutya - i-disodium-5-ribonucleotide (IMP + GMP), yamkelwe

Indlela yobude obubini [1]: bunzima i-0.4000 (m) g yesampulu, nyibilikisa kwaye ulungise umthamo ngamanzi

Ukuya kwi-250 ml, munca i-5.0 ml, kwaye ulungise umthamo nge-0.01 mol / L i-hydrochloric acid ukuya

I-250 ml, kunye ne-0.ol mol / L njengento engenanto, i-absorbeances A kunye ne-B kwi-250 nm kunye ne-280 nm zilinganiswe nge-cuvette ye-10 mm. I-Disodium-5-ribonucleotide (IMP + GMP) iqulethe

Ubungakanani bubalwa ngokwefomula (1).

ω Ngaba ipesenti yokufuma kwencasa ye-nucleotide disodium.

I-Sb / T 10371 isebenzisa indlela yobude obunye.]: Ubunzima 2 ~ 4G isampuli

(m) yachithwa ngexabiso elincinci le-0.01 mol / L i-hydrochloric acid kwaye igxininiswe kwi-100 ml,

Hlunga, munca i-5.00 ML ukuya kwi-100 ml yeflask yevolumetric kwaye usebenzise

I-0.01 mol / L i-hydrochloric acid ukuya kwivolumu. Sebenzisa i-10 mm cuvette, 0.01 mol / L

I-Hydrochloric acid isetyenziswe njengendawo engenanto ukulinganisa ukufunxa kwayo.

Ezi ndlela zimbini zisetyenziswa ngoku, kwaye akukho mda ongqongqo kwintsimi yesicelo

Umda. I-Sb / T 10371 yindlela yokufumanisa i-essence yenkukhu, ngokukodwa ejolise kuyo

Ukufunyanwa komxholo we-I + G: umxholo umalunga ne-1 ～ 39,6, kunye ne-QB / T 2845

Indlela yokufumanisa yezongezo ezicocekileyo ze-I + G, kodwa kwenye i-I + G equlathe

Ikwasetyenziswa kwiimveliso ezinobungakanani obuphantsi, njenge-GB / T 8967

Umxholo we-I + G kwioli esulungekisiweyo ilinganiswa ngokucaphula umxholo we-I + G kwi-QB / T 2845

Indlela yokubona. Ngenxa yale meko, umbhali uthathe inkuku njengesampulu wathelekisa ezi zimbini

Uphononongo lokuthelekisa ezi ndlela zimbini lwenziwa ukuphanda ukufana kwazo kunye nomahluko, ukuze kwenziwe uphononongo olongezelelweyo

Ibonelela ngereferensi yokwenziwa kwemigangatho yokumiselwa kwe-I + G.