hematoxylin bakeng staining

Hematoxylin Detailed selelekela:
et synonymes de: hematoxylin ; H (a) ematine
limolek'hule Mokgwa wa ho Nahana: C16H14O6
limolek'hule Weight: 302,28
Cas No .: 517-28-2

Qhibiliha Point: 200 ° C

Hematoxylin -eosin staining (hematoxylin-eosin staining), o ile a bua ka hore ke HE staining, parafini karolo thekenoloji atisa ho sebelisoa e 'ngoe ea mokhoa oa ho daea. Hematoxylin e alkaline, haholo-holo ka motheo oa chromatin le cytoplasm tsa ribosomes pherese putsoa; khubelu bakeng sa dae acid e, e cytoplasm khōlō le kantle ho sele popeho ka bophara e khubelu. O ILE A staining ke mokhoa tsa motheo le ho fetisisa ho pharaletseng sebelisitsoeng ka eona temaneng sobotta, embryology, bolwetse ho ruta le ho etsa lipatlisiso.

Dae tlhophiso:
The thepa tse bonolo ho e 'mala ke dae alkaline kapa asiti ba bitsoa basophilia le acidophilia, ha neutrophils le lidae acid e na le amana le fokolang ka neutrophilia.
The liprotheine ka dinama tse nyenyane tse ho etsa e ngata-amino acid ka protheine, 'me ba na le lintlha tse fapaneng isoelectric. Ka ho daea mokhoa tloaelehileng, e ph boleng ya daea mokelikeli e ka pH6, lintho tse intracellular asiti tse kang chromatin tsa khubu eo, kaba endoplasmic reticulum le 'mele Nissl ka lisele tsa tsona li tšoelesa le lisele tse methapo ea kutlo tse qaphalletsoa le lidae alkaline, bitsoa basophilic. Le liprotheine tse ling tse ka cytoplasm, tse kang lisele tse khubelu tsa mali ho haemoglobin, eosinophils likaroloana le collagen likhoele le likhoele mesifa li qaphalletsoa ke dae acid e, tsena dintho e tsejoang e le eosinophilic. Ha o fetola ph ya daea tharollo, eketseha ph boleng, tsa pele lidae acid e ka fetoloa dintho tsa alkaline basophilic; phokotso ph, le pele dyestuff daea tse bonahalang ho ka ba eosinophilic. E le hore le ph boleng ba tharollo staining ka ama daea itšoara joang.
Asiti Deoxyribonucleic (DNA) ka liketane a mabeli a phosphate sehlopha kantle, le qoso e mpe, asiti, ho ke ke ho le bonolo 'me ka sebele ba qosoa hematoxylin lidae le Ion oxyphobe le ile qaphalletsoa. Hematoxylin e putsoa ka tharollo alkaline, kahoo motheo e qaphalletsoa putsoa. Eosin Y (tetrabromofluorescein) ke lik'hemik'hale tsa maiketsetso acid e dae hore dissociates be anion hlonamisitsoe qosoa ka metsi le tlama ho ealogy sebele qosoa tsa protheine ea ho etsa hore cytoplasm, cytoplasm, erythrocyte, mesifa, connective lisele, eosinophils, joalo-joalo ke le dailoeng ka likhato e fapaneng ya khubelu kapa pinki, ka fapana le hoo ho ea nuclei putsoa. Euphorbia ke dae e ntle bakeng sa cytoplasm.
Ka lebaka la ho sebopeho sa tšoaneng tsa mokgatlo lisele tse ka hematoxylin amana le ho daea thepa ha e tšoanang. Mora hematoxylin staining, motheo le k'halsiamo letsoai mucus le putsoa, fumaneha hydrochloric dipharologano fa tse ling acid e joala le ba fokolang alkaline tharollo e ne e le 'mala o moputsoa, e kang ho phekola ho loketse, ho ka etsa hore motheo e hlakileng lefifi putsoa, cytoplasm le metsoako e meng decolorization. 'Me joale tshebediso ya cytoplasmic dae Euphorbia cytoplasm, e le hore metsoako e fapa-fapaneng ea cytoplasm le ho bontša ka botebo e sa tšoaneng ea pinki fapaneng. Kahoo mefuta e fapaneng ya dinama tse nyenyane tse, kapa li-cell dikarolo le diso tsa kakaretso sebopeho morphological ka di behilwe.
Staining diphetho:
khubung e qaphalletsoa le hematoxylin ka e khanyang e putsoa, lefufuru popeho, khalsiamo letsoai likaroloana ne lefifi putsoa, mucus ne bohlooho putsoa. The cytoplasm e qaphalletsoa le shades e fapaneng ya pinki ho pinki, le likaroloana eosinophilic ka cytoplasm ba bofubelu bo khanyang. Collagen faeba ne palo pinki, faeba rekere e ne e khanyang pinki, lisele tse khubelu ea mali e ne e le 'mala oa lamunu, protheine mokelikeli ne pinki.
The colouring e amana le mofuta oa dinama tse nyenyane tse, kapa li-cell, 'me hape e fetola ka lona potoloho bophelo le liphetoho pathological. Mohlala, lisele tse ngata ka nako nascent tsa cytoplasm ka 'mala o mofubelu ea khanya kapa hanyenyane basophilic, ha botsofali kapa holofatsang lona liphetoho tse eosinophilic staining. Collagen likhoele ka botsofali le sebeletsang pepeneneng denaturation, 'mala o mofubelu ho tloha tebang ho tebileng.

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