hematoxylin mo staining

Hematoxylin faatomuaga auiliili:
Upu e Uiga Tutusa: hematoxylin ; h (a) ematine
Fua Faatatau Molecular: C16H14O6
Weight Molecular: 302,28
CAS leai .: 517-28-2

Faaliusuavai Point: 200 ° C

Hematoxylin -eosin staining (staining hematoxylin-eosin), ua taua o HE staining, paraffin fuaiupu tekinolosi faaaoga masani i se tasi o auala dyeing. O Hematoxylin alkaline, e matele i le vaega faaogatotonu len¯ao o le chromatin ma le cytoplasm o le lanu moana pauli ribosomes; mumu mo le vali acid, o le cytoplasm autu ma Taiala extracellular i le tuufaatasiga o mumu. HE staining o le faavae ma o le tele auala faaaoga faalauaitele i histology, embryology, pathology aoaoga ma suesuega.

Dye faavasegaina:
O le meatotino e faigofie ona lanu e dyes alkaline po acidic ua valaauina basophilia ma acidophilia, ao vaivai maua neutrophils ma dyes acid affinity pei neutrophilia.
O le polotini i le pepa solo ai se tele o acids amino i le porotini, ma latou manatu isoelectric eseese. I le auala dyeing masani, o le TA taua o le dyeing suavaia e uiga i pH6, o le mea acidic intracellular e pei o le chromatin o le vaega faaogatotonu len¯ao, o le reticulum endoplasmic talatala ma le tino Nissl i le sela puna ma sela uaua ua pisia i dyes alkaline, valaauina basophilic. Ma isi polotini i le cytoplasm, e pei o le sela mumu toto i hemoglobin, eosinophils fasimea ma collagen alava ma alava musele ua pisia i dyes acid, o nei mea taua o eosinophilic. Afai e te suia le TA o le vaifofo dyeing, faateleina taua TA, o le dyes uluai acid e mafai ona liua i mea o alkalinity basophilic; TA faaitiitia, o le mea e uluai dyeing dyestuff mafai ona avea eosinophilic. Ina ia le TA taua o le vaifofo staining mafai ona aafia ai le tali dyeing.
Acid Deoxyribonucleic (DNA) i luga o le filifili e lua o le vaega phosphate fafo, faatasi ai ma se totogi lelei, acidic, e faigofie ma lelei molia dyes hematoxylin ma fusia Ion ma sa pisia. O lanu moana Hematoxylin i fofo alkaline, ina ua pisia lanu moana le vaega faaogatotonu len¯ao. Eosin Y (tetrabromofluorescein) o se chemically synthesized e vali acid dissociates i se anion molia le lelei i le vai ma e noatia i le puipuiga molia lelei o le porotini e faia le cytoplasm, cytoplasm, erythrocyte, maso, connective aano o le tino, eosinophils, ma isi o loo ua faalanu i vaega eseese o le mumu pe piniki, i se faatusatusaga maai i le nuclei lanumoana. O Euphorbia a vali lelei mo cytoplasm.
Ona o le tuufaatasiga eseese o le faalapotopotoga po o le sela i le hematoxylin affinity ma dyeing meatotino e le tutusa. Ina ua mavae staining hematoxylin, o le vaega faaogatotonu len¯ao ma kalisiu masima vavale ma isi lanu moana, o loo maua eseesega ava malosi acid hydrochloric ma fofo alkaline vaivai sa uli, e pei o le togafitiga talafeagai, e mafai ona faia le vaega faaogatotonu len¯ao manino pogisa lanu moana, cytoplasm ma isi vaega decolorization. Ma le faaaogaina o cytoplasm vali cytoplasmic Euphorbia, ina ia vaega eseese o le cytoplasm ma faaali a loloto eseese o eseese piniki. Ina ia mafai ona faaalia e le tele o pepa solo po o feaveai vaega ma manuʻa o le faatulagaga aoao morphological.
Staining taunuuga:
O le vaega faaogatotonu len¯ao ua pisia i hematoxylin i pupula lanu moana, o Taiala ivi, sa pogisa lanu moana kalisiu fasimea masima, vavale sa efuefu lanumoana. Ua pisia le cytoplasm ma shades eseese o lanu piniki e lanu piniki, ma fasimea eosinophilic i le cytoplasm E atamamai mumu. Collagen alava sa piniki sesega, sa alava ofuvae piniki susulu, sela mumu o le toto sa moli, sa piniki suavaia porotini.
E fesootai i le valivali i le ituaiga o pepa solo po o feaveai, ma e suia ai foi ma lona ola taamilosaga ma suiga pathological. Mo se faataitaiga, o le toatele sela i le vaitaimi nascent o cytoplasm i le lanu mumu o le malamalama po o le teisi basophilic, pe a lona soifua matua po o suiga degenerative e staining eosinophilic. Collagen alava i le matutua ma denaturation manino, o le lanu mumu mai papaʻu ona loloto.

Vaavaai mo lelei Hematoxylin mo Lab gaosi oloa & tuuina? Ua ia i tatou o se vaega filifilia lautele i tau tele e fesoasoani e te maua ai le foafoaga. Uma le Suafa Tutusa Haematine A Hematoxylin ua faamaonia lelei. O i tatou o Saina Amataga gaosi o Hematoxylin Faaaogaina mo Mordant Dye. Afai ei ai so o se fesili, faamolemole lagona saoloto e faafesootai i tatou.

Vaega faapitoa oloa: vailaau Inorganic