hematoxylin ngoba staining

Hematoxylin isingeniso onemininingwane:
Umqondofana: hematoxylin ; h (a) ematine
Formula Yamamolekhula: C16H14O6
Yamamolekhula Isisindo: 302,28
Cas No .: 517-28-2

Ukuncibilika Point: 200 ° C

Hematoxylin -eosin staining (hematoxylin-eosin staining), ukubizwa ngokuthi YENA staining, upharafini kwesigaba ubuchwepheshe elisetshenziswa kakhulu omunye indlela ukudaya. Hematoxylin kuyinto ane-alikhali, ikakhulu kuyi-nucleus ye-chromatin kanye cytoplasm we ribosomes onsomi okwesibhakabhaka; abomvu ukuze udayi acid, cytoplasm main futhi-matrix extracellular lichaza ekwakheni abomvu. YENA staining kuyinto inqubo eyisisekelo kunazo futhi iningi kabanzi olusetshenziswe Histology, Embryology, zokugula ukufundisa nokucwaninga.

Dye ngezigaba:
The izindawo ukuthi kulula ukuba yayithonywe udayi ane-alikhali noma esidi abizwa ngokuthi basophilia futhi acidophilia, kuyilapho ama-neutrophil futhi acid udayi babe ziyezwana buthakathaka njengamanzi neutrophilia.
Namaprotheni nezicubu bakha okuningi kwama-amino acid e amaprotheni, futhi amaphuzu ezahlukene isoelectric. Esikhathini abavamile ukudaya indlela, inani pH ketshezi ukudaya imayelana pH6, le intracellular izinto esidi ezifana chromatin ka-nucleus, emangelengele reticulum endoplasmic futhi umzimba Nissl kumaseli indlala futhi ezinzwa kuthiwa ingcoliswe udayi ane-alikhali, Ngokuthi basophilic. Futhi ezinye amaprotheni ku cytoplasm, ezifana abomvu egazi athwala umoya-mpilo, eosinophils izinhlayiya kanye collagen ethandele futhi imisipha imicu eminingana ongcoliswe udayi acid, lezi izinto ezaziwa ngokuthi eosinophilic. Uma ushintsha pH ikhambi ukudaya, pH value inyuka, original acid udayi zingaphendulwa zibe yizindawo yokuhlanganela izinto ka ayinayo basophilic; pH ukunciphisa, original mercury ukudaya impahla bangaba eosinophilic. Ngakho ukuthi inani pH yesixazululo staining kungathinta ukusabela ukudaya.
Deoxyribonucleic acid (DNA) kwi namaketanga amabili eqenjini phosphate kwangaphandle, nenkokhiso negative, ene-asidi, kulula futhi nakanjani icala hematoxylin udayi ne ion kunenze nisondelane futhi namabala. Hematoxylin siluhlaza e isixazululo ane-alikhali, ngakho-nucleus is namabala okwesibhakabhaka. Eosin Y (tetrabromofluorescein) kuyinto kwemiqondo amakhemikhali acid udayi ukuthi dissociates ibe anion omubi icala ngamanzi sibopha kuya seka nakanjani icala amaprotheni ukwenza cytoplasm, cytoplasm, erythrocyte, imisipha, izicubu zezinzwa, eosinophils, njll kukhona ezidayiwe e ngamazinga ahlukene ebomvu noma pink, okuphambene kakhulu nobuka blue nuclei. Euphorbia kuyinto udayi kuhle cytoplasm.
Ngenxa yenziwe ngohlobo oluhlukile kwenhlangano noma amaseli hematoxylin ziyezwana futhi ukudaya izakhiwo azifani. Ngemva hematoxylin staining, nucleus ne-calcium usawoti isikhwehlela nezinye okwesibhakabhaka, etholakalayo hydrochloric acid utshwala kwamangqamuzana futhi isixazululo ane-alikhali ibuthakathaka kwaba blue, ezifana ukwelashwa okufanele, ungenza i-nucleus ecacile luhlaza, cytoplasm nezinye izithako decolorization. Futhi ngaleso sikhathi ukusetshenziswa udayi cytoplasmic Euphorbia cytoplasm, ukuze izingxenye ezihlukahlukene cytoplasm nokubonisa a pink ezahlukene ukujula ezahlukene. Ngakho ezihlukahlukene izicubu noma cell izingxenye futhi izilonda jikelele isakhiwo morphological kungenziwa ukuboniswa.
Staining Imiphumela:
I-nucleus is ingcoliswe hematoxylin ku bright blue, uqwanga-matrix, i-calcium usawoti izinhlayiya babe luhlaza, isikhwehlela kwaba grey okwesibhakabhaka. I cytoplasm is ingcoliswe shades ezahlukene pink pink, futhi izinhlayiya eosinophilic ku cytoplasm igqamile abomvu. Collagen fibre kwaba pink okhanyayo, fibre nokunwebeka kwaba pink elikhanyayo, amangqamuzana egazi abomvu babe orange, amaprotheni ketshezi pink.
I Umbala ihlobene uhlobo izicubu noma cell, futhi sishintsha okukuyo umphefumulo wayo umjikelezo kanye nezinguquko sokugembula. Ngokwesibonelo, amangqamuzana amaningi esikhathini nascent ka cytoplasm phezu umbala obomvu yokukhanya noma kancane basophilic, lapho ukuguga noma esiwohlozayo yayo izinguquko staining eosinophilic. Imicu yotshani collagen ku denaturation ukuguga futhi esobala, umbala obomvu kusukela engajulile ukuze ezijulile.

Ufuna Hematoxylin ekahle Lab Umkhiqizi & umphakeli? Sine a Ukukhetha eziningi ngamanani ezinkulu ukukusiza uthole yendalo. Zonke Haematine Igama Same Njengoba Hematoxylin kuthiwa izinga yibhange. Singabantu China Umsuka Factory of Hematoxylin Isetshenziselwa Mordant Dye. Uma kukhona umbuzo, sicela ukhululeke ukuxhumana nathi.

Umkhiqizo Izigaba: Inorganic Amakhemikhali